In Act 1, Scene 5,
    Ladyis confident, decisive, and ruthless.  In this scene, she receives the letter fromthat
    acquaints her with the Weird Sisters' statements that he would become Thane of Cawdor and king,
    as well as the fact that he was shortly thereafter named Thane of Cawdor.  After she reads his
    letter, she immediately resolves that he shall be king: "Glamis, thou art, and Cawdor, and
    shalt be / What thou art promised" (1.5.15-18).  She initially worries that Macbeth's
    nature "is too full o' th' milk of human kindness / To catch the nearest way"
    (1.5.17-18).  In other words, she never doubts for a moment that Macbeth will be king; she only
    worries that he may be too gentle to be willing to killin order to hurry the process
    along. 
When she learns from a messenger that Duncan's retinue approaches,
    she calls his arrival at her home his "fatal entrance," letting us know that she has
    already, even at this early stage, conceived of a plan to have him killed so that Macbeth can
    take his place (1.5.46).  She then requests the assistance of those supernatural spirits that
    "That on mortal thoughts," saying
[...] unsex me
here,
And fill me from the crown to the toe top-full
Of direst cruelty. Make
thick my blood.
Stop up th' access and passage to remorse,
That no
compunctious visitings of nature
Shake my fell purpose, nor keep peace
between
Th' effect and it. (1.5.48-54)
 wants any
    nurturing, compassionate impulse of hers to be removed so that only her cruel and ruthless
    tendencies will remain.  She wants to make sure that she will feel no regret so that nothing in
    her womanly nature might dissuade her from the course of action on which she has resolved.  She
    requests that she be "unsex[ed]" so that she can be more like a man (or the way in
    which she and her society conceive of men to be): hard-hearted, implacable, and
    remorseless.
By Act 5, Scene 1, however, we see a very different Lady
    Macbeth.  It is clear that her earlier to become immune to "remorse" has not been
    granted.  As she sleepwalks, she is transported back in time to the night of Duncan's murder.
     She imagines that his blood is still on her hands, crying, "Out, damn spot, out, I
    say!" (5.1.37).  Though she said right after the actual murder that "A little water
    clears us of this deed," it is clear that she no longer believes it to be so easy to escape
    one's guilt (2.2.86).  Eventhat her servant brings to watch her recognizes that her "heart
    is sorely charged" (5.1.56-57).  Lady Macbeth clearly feels the heavy weight of
    self-reproach, and even the doctor knows he cannot help her because her ailment is not a
    physical one, but an emotional/spiritual one. 
In this scene, she recalls
    trying to force Macbeth to quickly move on from the guilt he felt immediately after the murder,
    saying, "What's done cannot be undone.  To bed, to bed, to bed" (5.1.70-71).  There
    was no point in regretting what they did then because there was nothing they could have done to
    change it.  By this time in the play, though, it is clear that Lady Macbeth has not successfully
    managed to keep regret away, that her weaker (and, to her, more feminine) impulses have overcome
    her desire to be ruthless, and her former decisiveness -- and unwillingness to consider any
    other course of action -- can now be blamed for her current, sad state.
It is
    notable, too, that in Act 1, Scene 5, Lady Macbeth speaks in(unrhymed iambic pentameter).  In
    Act 5, Scene 1, she speaks in prose.  Often, in Shakespeare's plays, when a noble character's
    speech changes from verse to prose, it is an indication that they have "gone mad."
     Such an interpretation certainly seems to fit here given Lady Macbeth's slipping grasp on
    reality and her later suicide.  Thus, we can also read this change in the way she speaks as
    further evidence of her character's transformation.
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